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Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis
Manage episode 453253622 series 3391756
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A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time.
Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy.
This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics.
In this episode of Listen In, hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity.
See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.
We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration.
You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems.
Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/
"Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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